Vibrational Force on Accelerating Orthodontic Tooth Movement: A Systematic Review and Meta-Analysis.

This study aimed to systematically gather and analyze the current level of evidence for the effectiveness of the vibrational force in accelerating orthodontic tooth movement (OTM). This systematic review was conducted using three electronic databases: Scopus, PubMed, and Google Scholar until March 2022. The search was done through the following journals: European Journal of Orthodontics, American Journal of Orthodontics and Dentofacial Orthopedics, The Angle Orthodontist, Progress in Orthodontics, and Seminars in Orthodontics. Human or animal studies that have evaluated the effect of vibrational force on the rate of OTM were selected. A meta-analysis was performed for the rate of canine movement per month. Database research, elimination of duplicate studies, data extraction, and risk of bias assessment were performed by authors independently and in duplication. A fixed and random-effect meta-analysis was performed to evaluate the effect of vibrational forces. A total of 19 studies (6 animal and 13 human studies) that met the inclusion criteria were included. Meta-analysis was performed based on four human clinical trials. Three out of four studies showed no significant difference in the rate of canine movement between vibrational force and control groups. The limitation of this study was the small sample size and significant heterogeneity among the studies. Although vibrational forces have been shown to accelerate OTM in experimental studies, the results are inconsistent in clinical studies. The inability to apply desired peak load to the targeted teeth may be the main factor in inconsistent clinical outcomes.

The effects of different types of periodontal ligament material models on stresses computed using finite element models.

INTRODUCTION: Finite element (FE) method has been used to calculate stress in the periodontal ligament (PDL), which is crucial in orthodontic tooth movement. The stress depends on the PDL material property, which varies significantly in previous studies. This study aimed to determine the effects of different PDL properties on stress in PDL using FE analysis. METHODS: A 3-dimensional FE model was created consisting of a maxillary canine, its surrounding PDL, and alveolar bone obtained from cone-beam computed tomography scans. One Newton of intrusion force was applied vertically to the crown. Then, the hydrostatic stress and the von Mises stress in the PDL were computed using different PDL material properties, including linear elastic, viscoelastic, hyperelastic, and fiber matrix. Young's modulus (E), used previously from 0.01 to 1000 MPa, and 3 Poisson's ratios, 0.28, 0.45, and 0.49, were simulated for the linear elastic model. RESULTS: The FE analyses showed consistent patterns of stress distribution. The high stresses are mostly concentrated at the apical area, except for the linear elastic models with high E (E >15 MPa). However, the magnitude varied significantly from -14.77 to -127.58 kPa among the analyzed patients. The E-stress relationship was not linear. The Poisson's ratio did not affect the stress distribution but significantly influenced the stress value. The hydrostatic stress varied from -14.61 to -95.48 kPa. CONCLUSIONS: Different PDL material properties in the FE modeling of dentition do not alter the stress distributions. However, the magnitudes of the stress significantly differ among the patients with the tested material properties.

Peak loads on teeth from a generic mouthpiece of a vibration device for accelerating tooth movement.

INTRODUCTION: The effect of vibrational force (VF) on accelerating orthodontic tooth movement depends on the ability to control the level of stimulation in terms of its peak load (PL) on the tooth. The objective of this study was to investigate the PL distribution on the teeth when a commercial VF device is used. METHODS: Finite element models of a human dentition from cone-beam computed tomography images of an anonymous subject and a commonly used commercial VF device were created. The device consists of a mouthpiece and a VF source. The maxilla and mandible bites on the mouthpiece with the VF applied to it. Interface elements were used between the teeth and the mouthpiece, allowing relative motion at the interfaces. The finite element model was validated experimentally. Static load and VF with 2 frequencies were used, and the PL distributions were calculated. The effects of mouthpiece materials and orthodontic appliances on the PL distribution were also investigated. RESULTS: The PL distribution of this kind of analyzed device is uneven under either static force or VF. Between the anterior and posterior segments, the anterior segment receives the most stimulations. The mouthpiece material affects the PL distribution. The appliance makes the PL more concentrated on the incisors. The VF frequencies tested have a negligible influence on both PL magnitude and distribution. CONCLUSIONS: The device analyzed delivers different levels of stimulation to the teeth in both maxilla and mandible. Changing the material property of the mouthpiece alters the PL distribution.

Immunogenicity of a recombinant Lactobacillus casei, surface-expressed H151P mutant of Clostridium perfringens epsilon toxin and its protective responses in BALB/c mice.

Epsilon toxin (Etx) is the most important virulence factor of type D C. perfringens in ruminants. The recombinant vaccines can be used against Etx intoxication. This study aimed to investigate the humoral immune responses of mice against a recombinant Lactobacillus casei which surface-expressed H151P mutant of Etx (L. casei-ε) after oral and parenteral immunization routes. The protective immunity was determined by challenge with trypsin-activated Etx. Higher humoral immune responses were seen in parenterally vaccinated mice with Freund's-adjuvanted L. casei-ε than non-adjuvanted and negative controls (P<0.05). In the oral immunized mice, L. casei-ε displayed a significant difference in IgG titres compared with the negative controls. Challenge results showed full protection of oral immunized mice against one and two MLDs, and partial protection against 10 MLD of the trypsin-activated Etx, whereas, the parenteral immunized mice only induced 75 % of protection against one MLD. This may be related to the appropriate immunity responses by L. casei-ε at the mucosal surfaces, which highlights the role of the oral immunization. Thus, L. casei-ε can be considered as an oral vaccine candidate against Etx intoxication and enterotoxaemia.

Analysis of developmental level of counties of Fars in terms of health infrastructure indicators using standardized scores pattern approach and factor analysis.

INTRODUCTION: It is necessary for planning in order to achieve optimal development, to have knowledge and understanding of the current situation. This identification requires separate areas of study into planning and assessing regions of each area with development indicators and analysis and ranking each area in terms of having gifts of development. The study also aims to analyze the development level of counties of Fars in terms of health infrastructure indicators using standardized scores pattern and factor analysis. METHODS: This is a descriptive and applied study, which has discussed the levels of 29 counties of Fars based on 10 health selected indicators using a standardized scoring model. Data were collected using a data collection form developed by the researchers through the Center of Statistics and Shiraz University of Medical Sciences. Results were analyzed using Excel and SPSS 19. RESULTS: Based on calculations according to standardized score and factor analysis methods, Shiraz and Rostam had the most and the least level between the other cities, respectively. Also development coefficient and operating score of the studied counties ranged from a maximum of 0.894 to a minimum of -0.941, and a maximum of 3.861 to a minimum of 2.001, respectively. DISCUSSION: There are relatively large differences between different counties in healthcare sector, and most studied counties in terms of healthcare sector indicators are not satisfactory. So planning how to allocate healthcare resources from policy makers to improve the studied counties is essential.